Accession Number:

ADA618875

Title:

Effect of Ibuprofen Dose on Platelet Aggregation and Coagulation in Blood Samples From Pigs

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Personal Author(s):

• Martini, Wenjun Z.
• Deguzman, Rodolfo
• Rodriguez, Cassandra M.
• Guerra, Jessica
• Martini, Angela K.
• Pusateri, Anthony E.
• Dubick, Michael A.

Report Date:

2015-03-01

Pagination or Media Count:

7.0

Abstract:

Introduction Ibuprofen is commonly used by Soldiers in the deployed environment. This study investigated its dose effects on in vitro coagulation. Methods Blood samples were collected from 4 normal healthy pigs and were processed to make platelet-adjusted 100 10expn 3micro L blood samples. Ibuprofen was added to the samples at doses of 0 micro gmL control, recommended oral dose 163 micro gmL, 1x, 2x, 4x, 8x, 10x, 12x, 16x, and 20x. Arachidonic acid or collagen-stimulated platelet aggregation was assessed at 15 minutes after the addition of ibuprofen. Coagulation was assessed with measurements of prothrombin time PT and activated partial thromboplastin time aPTT, and thrombelastography by Rotem. Results A robust inhibition of ibuprofen on arachidonic acid-induced platelet aggregation was observed at all doses tested. Collagen-stimulated platelet aggregation was inhibited to 71 5 and 10 5 of the control values at ibuprofen doses of 4x and 20x , respectively both p 0.05. No changes were observed in PT at any dose, but aPTT was prolonged at dose of 16x and 20x. Rotem measurements of coagulation time, clot formation time, maximum clot firmness, and A10 were compromised at dose 16x and 20x all p 0.05. Conclusion Ibuprofen inhibited platelet aggregation at recommended doses, but did not compromise aPTT or coagulation profile until at 16 times the recommended doses and higher. Further effort is needed to clarify whether there are different dose responses between human and pig blood samples in trauma situations.

Descriptors:

• *ANTIINFLAMMATORY AGENTS
• *BLOOD COAGULATION
• *BLOOD PLATELETS
• *DOSAGE
• PROTHROMBIN
• SAMPLING
• SWINE
• THROMBOPLASTIN

Subject Categories:

• Biochemistry
• Anatomy and Physiology
• Medicine and Medical Research
• Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE