In Vitro Evaluation of an Injectable Chitosan Gel for Sustained Local Delivery of BMP-2 for Osteoblastic Differentiation
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
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We investigated the effect of sustained release of bone morphogenetic protein-2 BMP-2 from an injectable chitosan gel on osteoblastic differentiation in vitro. We first characterized the release profile of BMP-2 from the gels, and then examined the cellular responses of preosteoblast mouse stromal cells W-20-17 and human embryonic palatal mesenchymal HEPM cells to BMP-2. The release profiles of different concentrations of BMP-2 exhibited sustained releases 41 for 2 ngmL and 48 for 20 ngmL, respectively from the chitosan gels over a three-week period. Both cell types cultured in the chitosan gels were viable and significantly proliferated for 3 days p 0.05. Chitosan gels loaded with BMP-2 enhanced ALP activity of W-20-17 by 3.6-fold, and increased calcium mineral deposition of HEPM by 2.8-fold at 14 days of incubation, compared to control groups initially containing the same amount of BMP-2. In addition, schitosan gels loaded with BMP-2 exhibited significantly greater osteocalcin synthesis of W-20-17 at seven days, and of HEPM at both 7 and 14 days compared with the control groups p0.05. This study suggests that the enhanced effects of BMP-2 released from chitosan gels on cell differentiation and mineralization are species and cell type dependent.
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