Accession Number:

ADA618457

Title:

Burn Enhances Toll-Like Receptor Induced Responses by Circulating Leukocytes

Descriptive Note:

Journal article

Corporate Author:

TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO

Report Date:

2012-04-30

Pagination or Media Count:

10.0

Abstract:

Burn and toll-like receptors TLR are associated with innate immune system activation, but the impact of burn on TLR-induced inflammation responses by circulating leukocytes is unknown. To study this, C57BL6 mice were subjected to burn 3rd degree, 25 TBSA or sham procedure and 1-7 days later blood was collected. Whole blood cell suspensions were incubated for 24 hr in the presence of zymosan TLR-2 agonist or LPS TLR-4 agonist. The blood cultures were responsive to TLR2 and TLR4-mediated activation, resulting in the production of IL-6, IL-10, IL-17, TNF- , MIP-1 , MIP-1 , KC and RANTES. TLR2-induced KC and MIP-1 production was greater in the burn group at 3-7 days post-injury, whereas IL-6, IL-10, KC and MIP-1 were greater for TLR4-induced activation after burn. In conclusion, circulating leukocytes were responsive to TLR-induced activation and TLR-mediated inflammatory responses were enhanced 3-7 days post-injury, as evidenced by increased production of these inflammatory mediators.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE