Accession Number:

ADA618416

Title:

Oxidative Lung Injury in Virus-Induced Wheezing

Descriptive Note:

Annual rept. 1 May 2013-30 Apr 2014

Corporate Author:

TEXAS UNIV MEDICAL BRANCH AT GALVESTON

Personal Author(s):

Report Date:

2014-05-01

Pagination or Media Count:

96.0

Abstract:

Over the past year we have focused on the role of the transcription factor Nrf2 in controlling expression of antioxidant genes in the lung of RSV-infected mice. In particular, we have shown that an Nrf2-inducing agent, BHA, can restore in part expression of the antioxidant genes SOD1 and catalase following RSV infection. We have also established a colony of Nrf2 KO mice and shown that lack of this transcription factor results in enhanced airway disease and viral replication in the lung. We have also identified a new strategy to increase level of Nrf2 expression in the lung by the use of adenovirus-associated vector 2 AAV2. We have also initiated a fast backcross breeding protocol to generate Nrf2 KO mice in the BALBc strain. We continue enrolling children with viral bronchiolitis and have validated a new FDA-approved Luminex xTAG Respiratory Viral Panel for the identification of co-infections and their role in mediating oxidative injury in infants. This panel has been designed to simultaneously probe for 12 viral targets in a single patient specimen RSVA, RSVB, Influenza A, Influenza A subtype H1, Influenza A subtype H3, Influenza B, PIV-1, PIV-2, PIV-3, hMPV, Rhinovirus, and Adenovirus. We have published a peer-reviewed paper in the Am J Physiology and a comprehensive review in Antioxidant Redox Signaling. Some of the data have been presented at the ESPID meeting in Milan, Italy.

Subject Categories:

  • Biochemistry
  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE