Challenge of N95 Filtering Facepiece Respirators with Viable H1N1 Influenza Aerosols (Postprint)
APPLIED RESEARCH ASSOCIATES INC PANAMA CITY FL
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Specification of appropriate personal protective equipment for respiratory protection against influenza is somewhat controversial. In a clinical environment, N95 filtering facepiece respirators FFRs are often recommended for respiratory protection against infectious aerosols. This study evaluates the ability of N95 FFRs to capture viable H1N1 influenza aerosols. METHODs. Five N95 FFR models were challenged with aerosolized viable H1N1 influenza and inert polystyrene latex particles at continuous flow rates of 85 and 170 liters per minute. Virus was assayed using Madin-Darby canine kidney cells to determine the median tissue culture infective dose TCID50. Aerosols were generated using a Collison nebulizer containing H1N1 influenza virus at 1 x 108 TCID50mL. To determine filtration efficiency, viable sampling was performed upstream and downstream of the FFR. RESULTS. N95 FFRs filtered 0.8-microns particles of both H1N1 influenza and inert origins with more than 95 efficiency. With the exception of 1 model, no statistically significant difference in filtration performance was observed between influenza and inert particles of similar size. Although statistically significant differences were observed for 2 models when comparing the 2 flow rates, the differences have no significance to protection. CONCLUSIONS. This study empirically demonstrates that a National Institute for Occupational Safety and Health-approved N95 FFR captures viable H1N1 influenza aerosols as well as or better than its N95 rating, suggesting that a properly fitted FFR reduces inhalation exposure to airborne influenza virus. This study also provides evidence that filtration efficiency is based primarily on particle size rather than the nature of the particles origin.
- Medical Facilities, Equipment and Supplies
- Protective Equipment