Accession Number:

ADA616635

Title:

Burn Wound gammadelta T-Cells Support a Th2 and Th17 Immune Response

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Report Date:

2014-02-01

Pagination or Media Count:

9.0

Abstract:

Major burn triggers immune dysfunction, which is associated with wound healing complications. Gamma- T-cells have been shown to be important in postburn inflammation and wound healing however, their cytokine phenotype at the burn wound site is unknown. C57BL6 male mice were subjected to a major burn 25 TBSA, third degree or sham treatment. At 3 hours, 3 days, and 7 days thereafter, skin samples were collected and subjected to dispase and trypsin digestion to isolate single cells. The cells were phenotyped and evaluated for cytokine profiles by flow cytometry. Th1 cells were defined as interferon IFN positive, Th2 cells were defined as interleukin IL-10 positive, and Th17 cells were defined as IL-17 positive. At 7 days after burn a shift toward Th2 and Th17 positive T-cells at the wound site was observed. Further analysis revealed that at 3-hour postinjury the percentage of T-cells positive for IFN , IL-10, and IL-17 were comparable between sham and burn skin samples. At 3 days and 7 days postinjury the percentage of cells positive for each cytokine increased however, the increase was significantly greater for IL-10 and IL-17, as compared with IFN ie, 9 20-fold vs 3-fold. Skin T-cells preferentially produced IFN 20, which was unaffected by burn injury. These data demonstrate that burn wound T-cells are activated for enhanced cytokine production and display a shift toward a Th2 andor Th17 phenotype. In contrast, burn wound T-cells were not activated for enhanced cytokine production.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Pharmacology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE