Engineering Replacement Tissues with Amniotic Stem Cells
Annual rept. 22 Sep 2011-21 Sep 2012
RHODE ISLAND HOSPITAL PROVIDENCE
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The loss of bulk tissues in limb injuries, including segmental defects, is unfortunately common and results in extensive, complex wounds characterized by loss of several tissue types including, bone, cartilage, and muscle. Treatment of extensive multi-tissue defects poses many reconstructive challenges notably suitable cell sources, supportive 3-dimensional 3-D, spatially relevant matrices, and the engineering of tissues with functional properties appropriate to their biomechanical demands. The creation of such tissue replacements can be considered under the term, functional tissue engineering. We confirmed our original hypotheses that amniotic stem cells, which are widely available, can differentiate into multiple tissues and that the ability of cells to self-assemble and self-sort in 3-D matrices creates spatially robust tissue aggregates. We then investigated the chondrogenic potential of MSCs isolated from the amniotic and chorionic membranes seeded in agarose by assessing responses to growth factor delivery and compressive loading on matrix production.
- Medicine and Medical Research