Coenzyme Q10: A New Treatment for Hemorrhagic Shock
Final rept. 1 Aug 2011-31 Jul 2014
KANSAS UNIV MEDICAL CENTER KANSAS CITY
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Purpose To examine the effects of ubiquinol reduced form of Coenzyme Q10 in leukocytes, lungs, diaphragm, and microcirculation following hemorrhagic shock HS Design Experimental. MethodsInstrumentation Anesthetized rats were bled to induce HS by removing 40 of the blood volume over 60 minutes. The rats were resuscitated with blood and lactated Ringer s solution, with or without ubiquinol, and monitored for 120 minutes. Lungs and diaphragm were excised and harvested for hydrogen peroxide H2O2 concentration and apoptosis analysis. Leukocytes were analyzed for mitochondrial superoxide O2 at baseline, end of shock, and 120 minutes following fluid resuscitation. In another set of experiments, leukocyte adherence and mast cell degranulation MCD was obtained. Vascular permeability was assessed and reactive oxygen species ROS in the venular walls were determined. Sample Sprague Dawley male rats were used and randomly assigned to the control or experimental groups. There were 10 rats per group in each set of experiments. Analysis Statistical significances were defined at a p 0.05. Various types of nonparametric statistics were used. Findings Mitochondrial leukocyte O2 in the control group increased and there was a 30 rise at the end of the experiment, as compared to ubiquinol group. Similarly lung and diaphragm apoptosis in the control group was significantly higher. Diaphragmatic H2O2 in the control group was also significantly higher than the ubiquinol group. There were significant differences in leukocyte adherence, the MCD index, vascular permeability and microcirculation ROS production between the control and the ubiquinol groups. Implications for Military Nursing Ubiquinol is a safe and easily administered supplement that prevents damage and reperfusion injury following HS. Attenuating damage to organs with the use of ubiquinol following HS could be used in military personnel.
- Medicine and Medical Research