Accession Number:

ADA616144

Title:

Time-Dependent Effects of Chlorhexidine Soaks on Grossly Contaminated Bone

Descriptive Note:

Journal article

Corporate Author:

ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX

Personal Author(s):

• Krueger, Chad A.
• Masini, Brendan D.
• Wenke, Joseph C.
• Hsu, Joseph R.
• Stinner, Daniel J.

Report Date:

2012-10-01

Pagination or Media Count:

6.0

Abstract:

Objective The purpose of this study was to quantify the reduction in the bacterial burden of grossly contaminated bone segments using different chlorhexidine CHL solutions. We hypothesized that 4 CHL would be the most efficient decontaminate. Methods Fifty four bone segments were harvested from fresh frozen porcine legs. Each specimen was dropped onto a Mueller Hinton medium that was inoculated with Staphylococcus aureus lux. These genetically engineered bacteria emit photons in propor tion to their number, allowing for quantification. The segments were retrieved after 5 seconds of exposure. Baseline imaging provided the initial bacterial load. An equal number of specimens were soaked in normal saline NS, 2CHL, or 4CHL. Specimen reimaging was completed at the 5 , 10 , 20 , 30 , and 60 minute marks.

Descriptors:

• *BONES
• *CONTAMINATION
• BACTERIA
• BASE LINES
• EFFICIENCY
• IMAGES
• PHOTONS
• REPRINTS
• STAPHYLOCOCCUS
• TIME DEPENDENCE

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE