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Mammary Cancer and Activation of Transposable Elements

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Annual rept. 1 Sep 2013-31 Aug 2014

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The purpose of this project is to investigate molecular events of the preclinical stages of mammary cancer, specifically, the intersection between the development of genome demethylation, retrotransposon transcriptional activity, and retrotransposon-driven transcription of cellular genes in an engineered mouse model of mammary cancer. During the last 12 months collection of material for planned molecular analyses and preparation of RNA and DNA for molecular library construction was completed. Construction of two replicates of the planned RNA libraries was completed in Australia, a third replicate was unable to be com-pleted due to RNA degradation in some samples. The data was forwarded to my collaborator Dr Edwards at Washington Uni-versity in St Louis, who has initiated library analyses. DNA was also forwarded to Dr Edwards who has commenced construc-tion of DNA libraries with preliminary analyses expected by the end of 2014 or early in 2015. A laboratory technician Tim Smith has been accepted as a PhD student at the University of Adelaide, and his project will be to complete the wet lab mo-lecular analyses and verification of in silico results with independent samples. We believe that the eventual findings will pro-vide insights into understanding the role of genome hypomethylation and expression of retrotransposons in cancer ontogeny, and may impact cancer prevention in the future.

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  • Medicine and Medical Research

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