Evolutionary Profiling of the Transcriptional and Post-Transcriptional Wiring of the Circadian Clock in Normal and Perturbed Conditions
Progress rept. 1 Apr-31 Jul 2014
MASSACHUSETTS UNIV AMHERST
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Justification of under-spending There was some delay in setting up the subcontract at Israel. Expenditures by the subcontract have not yet been fully processed. We had planned to complete the comparative sequencing dataset by June. Although the first libraries failed in late May. Our collaborator redid sample collection, RNA-Extraction and library construction. Sequencing data for the new libraries arrived on August 10. We are currently analyzing these datasets and preliminary results suggest that all libraries are usable. We also expect a charge from our colleague at the Hebrew University for the budgeted amount now that their libraries are producing good quality data. Results Behavioral analysis of fly species. As we described on our proposal we carried out behavioral analysis of the different fly species and their response to temperature changes. Towards this, we tested the circadian locomotor activity of eight different species of drosophila from different parts of the world, that have adapted to different environmental conditions in three different temperatures and compared the results to the well-studied Drosophila melanogaster. We kept the flies in 12 hours light 12 hours dark cycles and monitored the flies locomotor activity during seven days at 18C deg, 25C deg and 29C deg. In addition we transferred part of the flies from 25C deg to 18C deg and from 25C deg to 29C deg in the fourth day of the experiment. We have tested the influence of the temperature transfer in two time points flies were transferred either three hours after light on ZT3 or three hours after light off ZTl 5.