Accession Number:

ADA615421

Title:

Development of a Diagnostic Tool to Detect DNA Methylation Biomarkers for Early-Stage Lung Cancer

Descriptive Note:

Final rept. 15 Nov 2013-14 Nov 2014

Corporate Author:

PURDUE UNIV LAFAYETTE IN

Report Date:

2015-02-01

Pagination or Media Count:

6.0

Abstract:

Biomarkers based on DNA methylation patterns combine genetic inherited and epigenetic environmental manifestations of lung-cancer risk and have great promise in detecting early-stage lung cancer. The current approaches for finding novel methylation targets, however, either do not provide DNA base-pair resolution or are laborious to perform. This application overcomes these challenges by combining protein engineering and fluorescence spectroscopy to develop a highly sensitive molecular complex for mapping DNA methylation patterns. We have successfully produced the proposed molecular probes. The performance of the DNA methylation-recognition domain and our detection platform has been successfully validated. The developed probe is ready to be transitioned to mapping DNA methylation patterns within a gene locus. This technology will facilitate clinical screening for early-stage lung cancer patients.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE