Development of a Diagnostic Tool to Detect DNA Methylation Biomarkers for Early-Stage Lung Cancer
Final rept. 15 Nov 2013-14 Nov 2014
PURDUE UNIV LAFAYETTE IN
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Biomarkers based on DNA methylation patterns combine genetic inherited and epigenetic environmental manifestations of lung-cancer risk and have great promise in detecting early-stage lung cancer. The current approaches for finding novel methylation targets, however, either do not provide DNA base-pair resolution or are laborious to perform. This application overcomes these challenges by combining protein engineering and fluorescence spectroscopy to develop a highly sensitive molecular complex for mapping DNA methylation patterns. We have successfully produced the proposed molecular probes. The performance of the DNA methylation-recognition domain and our detection platform has been successfully validated. The developed probe is ready to be transitioned to mapping DNA methylation patterns within a gene locus. This technology will facilitate clinical screening for early-stage lung cancer patients.
- Medicine and Medical Research