A Versatile Model of Open-fracture Infection: a Contaminated Segmental Rat Femur Defect
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
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The purpose of this study was to refine an accepted contaminated rat femur defect model to result in an infection rate of approximately 50. This threshold will allow examination of treatments aimed at reducing infection in open fractures with less risk of type II error. Defects were created in the stabilised femurs of anaethetised rats, contaminated with staphylococcus aureus and then debrided and irrigated six hours later. After 14 days, the bone and implants were harvested for separate microbiological analysis. This basic model was developed in several studies by varying the quantity of bacterial inoculation, introducing various doses of systematic antibiotics with and without local antibiotics. This model of open fracture infection has been further refined with potential for local and systemic antibiotics. This is a versatile model and with the concepts presented herein, it can be modified to evaluate various emerging therapies and concepts for open fractures.
- Anatomy and Physiology
- Medicine and Medical Research