Acceleration of Regeneration of Large-Gap Peripheral Nerve Injuries Using Acellular Nerve Allografts Plus Amniotic Fluid Derived Stem Cells (AFS)
Annual rept. 1 Sep 2013-31 Aug 2014
WAKE FOREST UNIV WINSTON-SALEM NC SCHOOL OF HEALTH SCIENCES
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Digital gait analysis was used in rats to successfully assess the impact of sciatic nerve injury and to evaluate the time course of recovery of function. The first two groups of nerve repairs studied nerve autograft and acellular nerve allografts had similar outcomes in the rat. Functional recovery at 4 months post-injury repair was incomplete but did not differ significantly between nerve repair techniques. Cell seeding of acellular nerves was not successful using the described techniques. A microneedle vaccination array was employed to pierce the epineurium of the acellular nerve in hundreds of places and allow infiltration of cells. The outcomes of this technique are being analyzed. Nerves were harvested from Lewis rats for preparation of allografts for sciatic nerve repair, but the anatomic features of the nerve did not allow sufficient numbers of allografts to complete the proposed studies. Strain differences in nerve branching patterns rendered several of the nerves unsuitable for processing according to the vendor s protocol. Acellular nerve allografts from Sprague-Dawley rats were provided and will be used for the research studies. These nerves are incorporated into other rat strains without immunologic disturbances according to the vendor AxoGen.
- Medicine and Medical Research