The Breast Cancer DNA Interactome
Final rept. 30 Sep 2011-29 Sep 2014
PALO ALTO INSTITUTE FOR RESEARCH AND EDUCATION INC CA
Pagination or Media Count:
Gene transcription may be regulated by remote enhancer regions through chromosome looping. The role that higher order chromatin organization plays in cancer progression and metastasis is not yet fully understood. Our major goal has been to characterize physical interactions among selected breast cancer gene loci in normal and malignant mammary cell lines. We used IGFBP3, a gene that has been involved in breast cancer pathogenesis, as bait in a 4C-seq experiment comparing normal breast cells HMEC and two different breast cancer cell lines MCF7, an ER positive cell line and MDA-MB-231, a triple negative cell line. We found that in HMEC the genes BCAS1-4 breast carcinoma amplified sequence 1-4 were found in the top 10 most significantly enriched regions for interactions with IGFBP3. We also found EGFR epidermal growth factor receptor, a gene that has also been implicated in cancer, to interact significantly with IGFBP3 in all three samples. We also characterized IRAIN, a noncoding RNA important in long range interactions. These data suggest an important role for chromosomal interactions in the pathogenesis of breast cancer.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research