Effect of Coadministration of Vancomycin and BMP-2 on Cocultured Staphylococcus aureus and W-20-17 Mouse Bone Marrow Stromal Cells in Vitro
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
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In this study, we aimed to establish an in vitro bacteriumbone cell coculture model system and to use this model for dose dependence studies of dual administration of antibiotics and growth factors in vitro. We examined the effect of single or dual administration of the antibiotic vancomycin VAN at 0 to 16 micro gml and bone morphogenetic protein-2 BMP-2 at 0 or 100 ngml on both methicillin-sensitive Staphylococcus aureus and mouse bone marrow stromal cells W-20-17 under both mono- and coculture conditions. Cell metabolic activity, LiveDead staining, double-stranded DNA dsDNA amounts, and alkaline phosphatase activity were measured to assess cell viability, proliferation, and differentiation. An interleukin-6 IL-6 enzyme-linked immunosorbent assay ELISA kit was used to test the bone cell inflammation response in the presence of bacteria. Our results suggest that, when delivered together in coculture, VAN and BMP-2 maintain their primary functions as an antibiotic and a growth factor, respectively. Most interestingly, this dual-delivery type of approach has shown itself to be effective at lower concentrations of VAN than those required for an approach relying strictly on the antibiotic. It may be that BMP-2 enhances cell proliferation and differentiation before the cells become infected. In coculture, a dosage of VAN higher than that used for treatment in monoculture may be necessary to effectively inhibit growth of Staphylococcus aureus. This could mean that the coculture environment may be limiting the efficacy of VAN, possibly by way of bacterial invasion of the bone cells. This report of a coculture study demonstrates a potential beneficial effect of the coadministration of antibiotics and growth factors compared to treatment with antibiotic alone.
- Medicine and Medical Research