Autologous Minced Muscle Grafts: A Tissue Engineering Therapy for the Volumetric Loss of Skeletal Muscle
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
Pagination or Media Count:
Volumetric muscle loss VML results in a large void deficient in the requisite materials for regeneration for which there is no definitive clinical standard of care. Autologous minced muscle grafts MG, which contain the essential components for muscle regeneration, may embody an ideal tissue engineering therapy for VML. The purpose of this study was to determine if orthotopic transplantation of MG acutely after VML in the tibialis anterior muscle of male Lewis rats promotes functional tissue regeneration. Herein we report that over the first 16 wk postinjury, MG transplantation 1 promotes remarkable regeneration of innervated muscle fibers within the defect area i.e., de novo muscle fiber regeneration 2 reduced evidence of chronic injury in the remaining muscle mass compared with nonrepaired muscles following VML i.e., transplantation attenuated chronically upregulated transforming growth factor- 1 gene expression and the presence of centrally located nuclei in 30 of fibers observed in nonrepaired muscles and 3 significantly improves net torque production i.e., 55 of the functional deficit in nonrepaired muscles was restored. Additionally, voluntary wheel running was shown to reduce the heightened accumulation of extracellular matrix deposition observed within the regenerated tissue of MG-repaired sedentary rats 8 wk postinjury collagen 1 area sedentary vs. runner, 41 vs. 30, which may have been the result of an augmented inflammatory response i.e., M1 CCR7 and M2 CD163 macrophage expression was significantly greater in runner than sedentary MG-repaired muscles 2 wk postinjury. These findings support further exploration of autologous minced MGs for the treatment of VML.
- Anatomy and Physiology
- Medicine and Medical Research