Intramuscular Transplantation and Survival of Freshly Isolated Bone Marrow Cells following Skeletal Muscle Ischemia-reperfusion Injury
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
Pagination or Media Count:
BACKGROUND Delayed treatment cellular therapies offer an attractive means to treat extremity injuries involving acute skeletal muscle ischemia reperfusion injury IR. Bone marrow is a rich source of stem and progenitor cells with the potential to improve skeletal muscle regeneration. The extent to which bone marrow cells BMCs may be useful for IR is not known. The purposes of this study were twofold 1 to evaluate BMC survival following intramuscular injection 0, 2, 7, and 14 days after injury and 2 to determine whether BMCs improve functional recovery following IR. METHODS Magnetic-activated cell sorting was used to isolate lineage-negative Linj BMCs and enrich for stem and progenitor cells. To evaluate in vivo cell survival following IR, Linj BMCs were injected intramuscularly 0, 2, 7, and 14 days after IR, and bioluminescent imaging was performed for up to 28 days after cell injections. To assess their ability to improve muscle regeneration, intramuscular injections were performed 2 days after injury, and in vivo muscle function was assessed 14 days later. RESULTS Linj BMCs survived throughout the study period regardless of the timing of delivery. Intramuscular injection of Linj BMCs did not improve maximal isometric torque 300 Hz however, both saline-injected and Linj BMCY injected muscles exhibited an increase in the twitch-tetanus ratio, suggesting that damage incurred with the intramuscular injections may have had deleterious consequences for functional recovery. CONCLUSION Although BMCs injected intramuscularly survived cell transplantation, they failed to improve muscle function following IR. The ability of BMCs to persist in injured muscle following IR lends to the possibility that with further development, their full potential can be realized.
- Anatomy and Physiology
- Medicine and Medical Research