Oxygen Generating Biomaterials Preserve Skeletal Muscle Homeostasis under Hypoxic and Ischemic Conditions
ARMY INST OF SURGICAL RESEARCH FORT SAM HOUSTON TX
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Provision of supplemental oxygen to maintain soft tissue viability acutely following trauma in which vascularization has been compromised would be beneficial for limb and tissue salvage. For this application, an oxygen generating biomaterial that may be injected directly into the soft tissue could provide an unprecedented treatment in the acute trauma setting. The purpose of the current investigation was to determine if sodium percarbonate SPO, an oxygen generating biomaterial, is capable of maintaining resting skeletal muscle homeostasis under otherwise hypoxic conditions. In the current studies, a biologically and physiologically compatible range of SPO 1 2 mgmL was shown to 1 improve the maintenance of contractility and attenuate the accumulation of HIF1 , depletion of intramuscular glycogen, and oxidative stress lipid peroxidation that occurred following 30 minutes of hypoxia in primarily resting duty cycle 0.2 s train120 s contraction interval 0.002 rat extensor digitorum longus EDL muscles in vitro 95 N 5 CO2, 37 C 2 attenuate elevations of rat EDL muscle resting tension that occurred during contractile fatigue testing 3 bouts of 25 100 Hz tetanic contractions duty cycle 0.2 s2 s 0.1 under oxygenated conditions in vitro 95 O2 5 CO , 37 C and 3 improve the maintenance of contractility in vivo and prevent glycogen depletion in rat tibialis anterior TA muscle in a hindlimb ischemia model i.e., ligation of the iliac artery. Additionally, injection of a commercially available lipid oxygen-carrying compound or the components sodium bicarbonate and hydrogen peroxide of 1 mgmL SPO did not improve EDL muscle contractility under hypoxic conditions in vitro. Collectively, these findings demonstrate that a biological and physiological concentration of SPO 1 2 mgmL injected directly into rat skeletal muscle EDL or TA muscles can partially preserve resting skeletal muscle homeostasis under hypoxic conditions.
- Anatomy and Physiology
- Medicine and Medical Research