Bio-Physicochemical Interactions of Engineered Nanomaterials in in Vitro Cell Culture Model
Final rept. 15 Aug 2011 14 Aug 2014
INDIAN INST OF CHEMICAL TECHNOLOGY HYDERABAD
Pagination or Media Count:
The study in this project was undertaken to document the effect of 24 hours exposure of cerium oxide CeO2, cobalt oxide Co3O4 and tungsten oxide WO3 nanoparticles NPs on different cell lines. The study consisted of physicochemical characterization, cytotoxic, genotoxic and oxidative stress responses due to in vitro exposure to different doses of NPs and their microparticles MPs. The physicochemical properties like size, size distribution, state of dispersion and zeta potential of these NPs are the important factors to study their toxicity. To investigate the potential role of oxidative stress as a mechanism of toxicity, reactive oxygen species ROS, nitric oxide NO, lactate dehydrogenase LDH level and reduction in the total glutathione GSH were estimated. The results showed that, there was significant induction of ROS, NO, LDH and GSH level observed in IMR32 cells treated with CeO2 NPs from 100 gml onwards, whereas in their bulk no significant changes were observed. Similarly, HEK293 cells treated with Co3O4 NPs showed significant increase in ROS, membrane leakage of LDH and NO and reduction in the total GSH at the dose level of 50 gml onwards however, bulk were not found to induce any significant changes. Comet assay of the NMs and bulk treated cells showed a concentration dependent increase in the percentage of tail DNA, however only the NMs showed a significant increase.