Accession Number:

ADA614415

Title:

Imaging Prostate Cancer Microenvironment by collagen Hybridization

Descriptive Note:

Annual rept. 30 Sep 2013-29 Sep 2014

Corporate Author:

JOHNS HOPKINS UNIV BALTIMORE MD

Personal Author(s):

Report Date:

2014-10-01

Pagination or Media Count:

8.0

Abstract:

We continue to derivatize and test the collagen mimetic peptides CMPs for imaging. We continue to work on 1 Dual radio- and fluorescent labeling of CMPs retaining high-affinity and specificity for intact and digested collagen type I films 2 Validation of dual-labeled CMPs that display high affinity and specificity for stromal collagens in frozen PCa xenografts and, 3 Measurement of pharmacokinetics and in vivo imaging of dual-labeled CMPs in mouse subcutaneous PC-3 xenograft and pancreatic xenograft models. Efforts have been focused on achieving a biologically stable CMP with both gamma-emitting and fluorescent labels. We have employed several strategies utilizing the previously biologically validated, high affinity basic CMP peptide core, which may contain chelators, radioiodine moieties and fluorescent dyes. While the radiochemistry aspect of the project has proved challenging, we have made substantial progress biologically with the optical agents as detailed below.

Subject Categories:

  • Biochemistry
  • Medicine and Medical Research
  • Radiobiology
  • Pharmacology
  • Radiation and Nuclear Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE