Understanding Tumor Dormancy as a Means of Secondary Prevention
Annual rept. 15 Sep 2013-14 Sep 2014
COLD SPRING HARBOR LAB NY
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The purpose of this collaborative project is to establish a molecular definition of the dormant state of a cancer cell. In doing this we hope to understand how this dormancy is broken, ultimately leading to recurrence in a patient that was stably in remission. Once our understanding of this is more complete it is hoped that we can devise strategies for secondary preventions. This funding year we have successfully optimized the two-photon microscopy for the singe cells from a tissue section and have established how to use the current technology to extract RNA and make high quality transcriptome libraries from lo w input materials. We anticipate additional advances in the quality of the transcriptome libraries from a single cell, fixed in PFA, as new technology is anticipated to be released before the end of the year. We have also made progress on strategies for identifying dormant cells in human biopsies.
- Anatomy and Physiology
- Medicine and Medical Research