Renewable Bio-Solar Hydrogen Production: The Second Generation (Part C)
Final rept. 1 Sep 2011-31 Aug 2014
COLORADO SCHOOL OF MINES GOLDEN
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Efforts in this project focused on H2biofuels production and metabolism in eukaryotic algae and cyanobacteria. These research efforts led to over thirty-four publications in the three-year project performance period, including publications in the top tier journals Nature Communications and Science. A principal focus in this iteration of research was the development of new model systems for algal H2 and biofuels production. This led to the genome sequencing of Nannochloropsis gaditana and the development of genetic transformation protocols for this algal. These results were published in Nature Communications and this alga is poised to become a new biotechnologically relevant model system. We also successfully developed fatty acid and terpenoid biosynthesissecretion modules in the cyanobacterium Synechococcus sp. PCC 7002. This very promising approach allows the transformation of sunlight to biofuels that are secreted and do not require costly phototroph harvesting, dewatering and deconstruction steps to obtain the biofuel precursors.