Functional Assessment of the Role of BORIS in Ovarian Cancer Using a Novel in Vivo Model System
Annual rept. 30 Sep 2013-29 Sep 2014
NEBRASKA UNIV AT OMAHA
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The purpose of this study is to determine the functional role of the putative oncogene BORIS in ovarian cancer. The proposed studies will use a newly developed mouse model system to assess the oncogenic potential of BORIS expression in the ovarian surface epithelium, alone and in combination with Rb and p53 knockout. In the past year we have accomplished a number of objectives i we have obtained IACUC, ACURO, and Biosafety approvals for the proposed studies, ii we designed and constructed a new conditional overexpression construct iZEG-CTCFL to drive BORIS expression in mice, iii we obtained double conditional knockout mice Rb, p53 in FVBN background and bred with FVBN wild-type mice, obtained F1 mice and intercrossed to generate stocks of single Rbflox or p53flox mice. iv we obtained founder BORIS-Tg mice and are currently crossing into the FVBN strain to fully characterize the transgenic gene configuration, v we conducted intrabursal Ad-Cre injections to generate cohorts of conditionally deleted Rb and p53 in mice, measured ovarytumor volume over 300 days by MRI, and confirmed that double mutant mice have significantly enhanced ovarytumor growth, and vi Ms. March has learned how to isolate and culture OSE cells from mice and has begun to isolate and utilize these cultures to meet the study objectives.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research