Axon-Sorting Multifunctional Nerve Guides: Accelerating Restoration of Nerve Function
Annual rept. 30 Sep 2013-29 Sep 2014
GENEVA FOUNDATION TACOMA WA
Pagination or Media Count:
Task 1 Engineer Laminin-collagen IV-entactin-perlecan LCEP binding domains as fusions to the growth and neurotrophic factors protein factors . Generate LCEP composite fiber scaffolds incorporating protein factors exhibiting either constant surface density or longitudinal density gradients. Match fiber stiffness with that of autograft G1 MPa. Characterize the bioactivity of scaffold-bound protein factors using cell signaling assays 0-15 months performance site USMA. Task 2 Evaluate each protein factor singly in selected combinations in the organotypic model system for preferential sensory or motor axon extension. Use confocal microscopy to track axon extension of labeled sensory or motor neurons from spinal cord slices motor or dorsal root ganglia DRG sensory. 20 Thy1-YFP mice will be used for this task and subtasks. 12-24 mo. sites JHU USMA Task 3 Evaluate top performing compositions from Task 2 in a rat femoral nerve repair model in which sensory and motor axons intermingle proximally and are segregated into discrete cutaneous and muscle branches distally. 80 Sprague-Dawley rats will be used for this task and subtasks. 24-36 mo. work performed at JHU.
- Anatomy and Physiology
- Medicine and Medical Research