Acute Inhalation Toxicity and Blood Absorption of 2,4-Dinitroanisole (DNAN) in Rats
Technical rept. Nov-Dec 2011
ARMY PUBLIC HEALTH COMMAND ABERDEEN PROVING GROUND MD ARMY INST OF PUBLIC HEALTH
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This toxicology study was conducted to determine the 4 -hour inhalation median lethal concentration LC50 of 2,4-Dinitroanisole DNAN in male and female rats . Nose-only exposure to the highest-achievable aerosol atmosphere of DNAN 2,4 mgL did not induce any compound-related mortality, adverse toxic signs, body weight changes, or gross necropsy findings. A secondary objective was to determine the effect that two different routes of administration inhalation and oral had on the absorption of the chemical into the bloodstream . Blood samples were collected and analyzed from exposed rats at 7 time points for those exposed via inhalation and 6 different time points for those given a calculated equivalent oral dose. The results of the blood absorption study indicated that, under the stated study conditions and limitations, acute exposure to DNAN via oral gavage appears to induce higher DNAN whole blood concentrations in laboratory rats compared to those exposed via inhalation. Blood concentrations of the metabolite 2,4-Dinitrophenol DNP were not different between the two exposure routes.
- Anatomy and Physiology
- Organic Chemistry