Mammary Cancer and Activation of Transposable Elements
Annual rept. 1 Sep 2013-31 Aug 2014
WASHINGTON UNIV ST LOUIS MO
Pagination or Media Count:
The purpose of this project is to investigate molecular events occurring in the preclinical stages of mammary cancer. Specifically, the project investigates the intersection between the development of genome demethylation, retrotransposon transcriptional activity, and retrotransposon-driven transcription of cellular genes in an engineered mouse model of mammary cancer. During the last 12 months, my collaborator Dr. Peaston completed the mouse breeding for the project, and collecting material for planned molecular analyses is now complete. We have received DNA for all replicates. We have made and performed initial of sequenced methylation profiling libraries for the first replicate and are in the process of making libraries for the others. and are currently making methylation profiling libraries. We have continued to make improvements to our methodologies for integrating and interpreting genome-wide expression and methylation data that will be used for the analyses in this project. We believe that the eventual findings will provide insights into understanding the role of genome hypomethylation and expression of retrotransposons in cancer ontogeny, and may impact cancer prevention in the future.
- Medicine and Medical Research