Intracellular Protein Delivery for Treating Breast Cancer
Final rept. 15 may 2011-14 May 2014
UNIVERSITY OF SOUTHERN CALIFORNIA LOS ANGELES
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Encapsulating anticancer protein therapeutics in nanocarriers is an attractive option to minimize active drug destruction, increase local accumulation at disease site and decrease side effects to other tissues. In this final report, we summarize major accomplishments for the study of polymeric nanocapsulses for delivering protein drugs apoptin and p53. We succeeded in synthesizing degradable, core-shell apoptin and p53 nanocapsules. Recombinant apoptin was reversibly encapsulated in a positively charged, water soluble polyermic shell and is released in native forms in responses to reducing conditions such as the cytoplasm. Rationally designed non-covalent protein nanocapsules, incorporating copper-free click chemistry moiety, PEG unit, redox-sensitive crosslinker, and tumor specific targeting ligand, were synthesized and were shown to selectively deliver intracellular p53 into tumor cells. The study validate a general approach for protein delivery into tumor cells for breast cancer treatment.
- Medicine and Medical Research