Accession Number:

ADA613664

Title:

Conjugation of the Dark Quencher QSY 7 to Various Synthetic Cannabinoids for Use in Fluorescence-Based Detection Platforms

Descriptive Note:

Final rept. Jan-Mar 2014

Corporate Author:

ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD WEAPONS AND MATERIALS RESEARCH DIRECTORATE

Report Date:

2015-02-01

Pagination or Media Count:

26.0

Abstract:

We have been able to synthesize and characterize several synthetic cannabinoid-dark quencher DQ conjugates for the use in fluorescence-based cannabinoid detection platforms. The carboxylic acid reactive QSY 7 amine was reacted with the carboxylated metabolites of JWH 018 and JWH 073 via a simple peptide conjugation reaction with the catalyst O-benzotriazole-N,N,N,N-tetramethyl-uronium-hexafluoro-phosphate to yield 3 new DQ conjugates. Thin layer chromatography and liquid chromatography coupled mass spectrometry show full conversion of the synthetic cannabinoid starting material to product with observed and expected masses of 1,097 gmol for JWH 018 pentanoic acid QSY 7 amine and 1,081.4 gmol for JWH 073 butanoic acid-QSY 7 amine. These characterized conjugates are now ready for cannabinoid-receptor-based binding assays.

Subject Categories:

  • Biochemistry
  • Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE