Accession Number:

ADA612784

Title:

Carbohydrate Nanotechnology: Hierarchical Assemblies and Information Processing from Oligosaccharide-Synthetic Lectin Host-Guest

Descriptive Note:

Final rept. 1 Jul 2013-30 Jun 2014

Corporate Author:

MIAMI UNIV CORAL GABLES FL

Report Date:

2014-09-17

Pagination or Media Count:

31.0

Abstract:

The information capacity of oligosaccharides greatly exceeds that of oligonucleotides or oligopeptides, but this property of carbohydrates is rarely accessed to create synthetic nanosystems. This project sought to use the information carrying capacity of oligosaccharides for creating hierarchical assemblies and for molecular information processing. Specifically, we have created new methods for printing multivalent carbohydrates with sub-micrometer feature diameters using a photochemically-controlled grafted-from brush polymerization strategy, and the resulting glycopolymers are orders-of-magnitude more sensitive towards lectins compared to their monomeric counterparts. In addition, we have expanded the surfaces that can be used to immobilize biological ligands to include graphene by developing a force-accelerated printing strategy, and in doing so, we have increased the understanding of force on surface reactions. Finally, we have developed a new, mannose-specific synthetic lectin that achieves selectivity using biomimetic cooperative and multivalent binding modes.

Subject Categories:

  • Biochemistry
  • Bionics

Distribution Statement:

APPROVED FOR PUBLIC RELEASE