Toward an Inexpensive Test for Vitamin D Levels in Blood
Annual rept. 28 September 2012-27 September 2013
TRUSTEES OF COLUMBIA UNIV NEW YORK
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The objective of the research is to test the feasibility of a vitamin D biosensor based on a selective redox enzyme immobilized onto an electrode. In long-term, this will make it possible to create a technology akin to commercial glucose sensors for in-clinic use as part of routine examinations by both reducing the cost of testing vitamin D levels and reducing the time for testing to less than a few minutes. For this purpose, a synthetic gene for recombinant human CYP27B1 the enzyme which is involved in vitamin D metabolism was designed. The enzyme was expressed in E. coli and the activity of this enzyme was verified spectrophotometrically. Finally, a synthetic redox mediator phenosafranine was found to be successful in mediating electron transfer between electrode and CYP27B1. This is important for introducing a way to electrochemically measure 25OHD circulating form of vitamin D in blood levels.
- Medicine and Medical Research