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Novel Therapy for Bone Regeneration in Large Segmental Defects

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Annual rept. 30 Sep 2013 - 29 Sep 2014

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The purpose of this study is to test the efficacy of thrombopoietin TPO to heal a segmental bone defect SBD in a large animal model, the minipig. The scope of the research comprises the following specific aims i to determine the union rate of tibial midshaft defects in minipigs treated with BMP-2, TPO, or saline control and ii to evaluate the safety and side effects of treating tibial midshaft defects in minipigs treated with BMP-2, TPO, or saline control. In Year 1 we completed a pilot study to work out the surgical and equipment logistics and have completed 12 minipig surgeries plus the 4 pilot minipig surgeries. We have collected baseline blood for analyses and have collected blood and xrays as per IACUC and ACURO approvals. At the time of this report only the first minipig was 1 month post-surgery a control and as expected, no evidence of bone union was observed. As we have just begun the minipig surgeries and our protocol is for the minipigs to be euthanized 1 year post-surgery for detailed bone analysis and the majority of blood biochemistries will be completed together at the end of the study, the bulk of our findings will be determined in the last year of the award Year 3. Thus, the most significant findings during this period are the large difference observed in the tibial geometry between the strains of minipigs we evaluated Ossabaw, Sinclair, and Yucatan, the different issues needed to be resolved to optimize the surgical procedure and post-op care, and the observation that for removing a significant piece of the tibia, there is not as much bleeding as would be observed with humans.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

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