Center for Regenerative Biology and Medicine at Mount Desert Island Biological Laboratory
Annual rept. 1 Jun 2011 - 31 May 2012
MOUNT DESERT ISLAND BIOLOGICAL LAB SALISBURY COVE ME
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The overarching goal of this proposal is to identify the regulatory networks controlling the patterned regrowth of damaged limbs and limb tissues in vertebrates, and gain insight into the fundamental genetic circuitry directing this complex process. During this 2011-2012 grant year, we have achieved two major goals in our research program. We have performed transcriptome analysis of salamander and Polypterus regenerating limbs and identified extracellular matrix ECM genes as the most highly upregulated pathway in response to injury. The ECM has been linked to cellular migration but the potential impact during the initiation of regenerative outgrowth, however, remains largely unknown. In addition, we have submitted RNA samples for deep-sequencing experiments to elucidate the contributions of microRNAs as key genetic determinants of positional memory during zebrafish appendage regeneration. We anticipate these microRNAs experiments to yield candidate factors for functional characterization for the duration of the grant period. In summation, we have made notable progress in our efforts to identify the common regulatory pathways required for appendage regeneration and remain on schedule to complete future milestones for grant year 2012-2013.
- Medicine and Medical Research