Genetic Networks Activated by Blast Injury to the Eye
Annual rept. 15 Jul 2013-14 Jul 2014
EMORY UNIV ATLANTA GA
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Purpose The present research project is designed to define the overall change in gene expression in the eye following a blast injury to the eye. In this process the genetic networks activated by injury will be defined along with biological markers of retinal injury. ScopeThe proposal will examine the changes in gene expression that occur in a mouse genetic reference panel, the BXD recombinant inbred Rl strain set. This analysis will define genomic loci modulating the response of the eye to a blast injury and the genetic networks activated by the injury. Major FindingCollected retinas from 40 normal strains with 148 microarrays run. We have collected phenotypic data on corneal thickness, lOP and lens opacity on 27 strains of normal mice and 27 strains following a 50psi blast injury. There was no difference in corneal thickness 5 days following the blast injury. We have added 8 strains 32 microarrays to the TATRC Normal Retinal Database that contains 148 microarrays from 38 normal strains. We have opened the DoD TATRC Normal Retina Database to the public at GeneNetwork.org. We have identified SOX11 as a good marker for neuronal injury in the retina and the manuscripts describing these results are being completed for publication. In January, the lab was moved to Emory University. We have constructed a new blast gun for use at Emory. With Dr. Mike Iuvone we have characterized the injury created by this blast publication in preparation. We have all animal protocols in place and have established a colony of BXD mice to use in the remaining portions of the projects.
- Genetic Engineering and Molecular Biology
- Anatomy and Physiology
- Medicine and Medical Research