Use of the Photo-Electromyogram to Objectively Diagnose and Monitor Treatment of Post-TBI Light Sensitivity
Annual rept. 6 Sep 2012-5 Sep 2013
IOWA UNIV IOWA CITY
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Purpose to test the whether photosensitivity photophobia after traumatic brain injury TBI is due to increased sensitivity of the brainstem trigeminal sensory nucleus, as revealed objectively by an exaggerated photoblink reflex photo-electromyogram. This will be tested in humans and in a mouse strain genetically engineered to be hypersensitive to calcitonin gene related peptide CGRP, the neurotransmitter modulating trigeminal nerve function. Scope objective methods to quantify photo-sensitivity include 1 light evoked potentials electromyogram from the blinking and squinting muscles of the forehead 2 the pupil light reflex 3 light evoked changes in sympathetic nerve activity, measured by changes in skin conductance and heart rate. Major Findings Year 2 1 First successful recording from EMG electrodes implanted into the mouse orbicularis oculi muscle with wireless transmission of EMG in response to stimuli in an awake, unanesthetized mouse, 2 Observation of increased orbicularis EMG activity in response to increased light levels, 3 Observation of an apparent enhancement of an air puff induced blink by light after administration of an intraperitoneal injection of calcitonin gene related peptide CGRP. Significance objective testing of photosensitivity in humans and mice will provide new approaches to finding the underlying mechanisms, classification of photosensitivity, diagnosis and monitoring of new treatments.
- Anatomy and Physiology
- Medicine and Medical Research