Portable Low-Volume Therapy for Severe Blood Loss
Annual rept. 09 May 2013-08 May 2014
MINNESOTA UNIV DULUTH DEPT OF BIOLOGY
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In our Year 2 Annual Report, we reported experiments described in Specific Aim 2. These included determining if outcomes from hemorrhagic shock in rats can be improved by combining BHBM with the proven hypothermia-promoting adjunct 3- iodothyronamine T1AM. This year, we explored the feasibility of administering a larger volume of a lower molarity BHBM to hemorrhagically shocked rats as described in Specific Aim 3. Results from these experiments are described in this Annual Report for Year 3. In summary we made the following conclusions 1.Melatonin provides therapeutic effects at very low concentrations. This was shown by the low histopathological score observed when administering a solution containing melatonin at a concentration 10-6-fold lower than that published by Klein et al 3. 2.An isotonic form of BHBM is not efficient for sustaining survival when compared to LR. Lactated Ringers LR administration is more efficient at sustaining survival after 60 blood loss both at 24 hours and 10 days after surgery than any other treatment. LR provides essential mineral constituents of blood that isotonic BHBM does not. 3. 10-day survivors are on their way to full recovery. The low histopathological scores for all the treatments administered suggest that if an animal has made it to 10 days it is not likely to develop multiple organ failure and die after the endpoint of our experiment.
- Medicine and Medical Research