The Impact of Epithelial-Stromal Interactions on Human Breast Tumor Heterogeneity
Annual rept. 15 Sep 2013-14 Sep 2014
ROYAL INSTITUTION FOR THE ADVANCEMENT OF LEARNING MONTREAL (CANADA)
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Heterogeneity is a key factor underlying the variability in patient response to treatment, especially in Triple-Negative TN breast cancer cases. In addition to the intrinsic molecular characteristics of the tumor epithelium, we and others have demonstrated that the breast stroma can influence breast cancer progression and response to therapy. Thus, the purpose of this project is to identify, define and formally test critical pathways mediating tumor epithelial-stromal communication and co-dependency in TN breast tumor subtypes associated with poor outcome. Our specific aims are to develop coordinate stromal-epithelial expression signatures and gene interaction networks, and to identify and integrate stromalepithelial and miRNA signatures associated with TN breast tumors. In pursuit of these goals, we have successfully isolated RNA and miRNA from patient epithelial and stromal tissues for gene expression analysis and miRNA profiling, and we are now positioned to discover relevant tumor-associated changes and epithelial-stromal gene expression networks as well as miRNA signatures associated with TN breast cancer.
- Medicine and Medical Research