Accession Number:

ADA612229

Title:

Induction of Food Allergy in Mice by Allergen Inhalation

Descriptive Note:

Annual rept. 30 Sep 2013-29 Sep 2014

Corporate Author:

CINCINNATI FOUNDATION FOR BIOMEDICAL RESERACH AND EDUCATION OH

Personal Author(s):

Report Date:

2014-10-01

Pagination or Media Count:

10.0

Abstract:

The purpose of this project is to test the hypothesis that food allergy may develop in response to antigen inhalation. Studies in a mouse model are determining the importance of 1 lung inflammation 2 dose of inhaled antigen 3 interactions among inhaled antigens and 4 the relative timing of antigen ingestion vs. antigen inhalation to lead to food allergy development. We are also testing whether exposure to aerosolized antigen will reverse or exacerbate established food allergy to that antigen. Studies in year 1 of this project demonstrate that 1 initial inhalation of the potent aeroallergen, house dust mite HDM, along with egg white EW, increases the severity of allergic airway disease that develops to further inhalation of EW, but does NOT food allergy induced by EW ingestion 2 intravenous injection of mice with IgE antibody to trinitrophenyl TNP, followed by oral challenge with TNP-bovine serum albumin TNP-BSA induces shock hypothermia, but not diarrhea 3 repeated injection of IgE anti-TNP, followed by challenge with TNP-BSA causes loss of the hypothermia response and generation of an IgG anti-TNP antibody. Finally, we have generated large quantities of monoclonal antibodies to IL-10R, TGF-beta and CD25, which will be used in our future studies. These studies can suggest improved strategies for preventing food allergy development, and possibly, for reversing established food allergy.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research
  • Food, Food Service and Nutrition
  • Toxicology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE