Radiolabeled Exosomes for the Early Detection of Metastases and to Predict Breast Cancer Premetastatic Niche
Annual rept. 1 Aug 2013-31 Jul 2014
CORNELL UNIV MEDICAL COLL (WEILL) NEW YORK
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Since current diagnostic tools fail to detect breast cancer BC metastatic spread at a very early stage, we proposed a new approach for the early detection of metastatic disease in BC patients using cancer cell derived particles known as exosomes as a guide. We hypothesized that exosomes tagged with appropriate radionuclides could be used as molecular imaging probes to study and non-invasively image metastatic spread, and to detect pre-metastatic niches using single-photon emission computed tomography SPECT or Positron emission tomography PET. During this report period we have 1 isolated and characterized exosomes from a series of BC cell lines of different metastatic capacity and tropism, 2 radiolabeled isolated BC exosomes with different radionuclides, such as 111In, 64Cu, 89Zr . We plan to 3 study the uptake and subsequent intracellular transport of radiolabeled exosomes by different cell types involved in premetastatic niche formation, 4 to study the in vivo stability, pharmacokinetics and biodistribution of radiolabeled exosomes following intravenous administration in naive and pathological mouse models. 5 Study the biodistribution and tumor uptake of radiolabeled exosomes derived from peripheral blood of BC patients in BC.
- Medicine and Medical Research