Identifying microRNAs that Regulate Neuroblastoma Cell Differentiation
Annual rept. 1 Sep 2013-31 Aug 2014
TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO
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We identified 14 microRNA candidates that induce neuroblastoma cell differentiation based on a high-content screening of neurite outgrowth the morphological differentiation marker of neuroblastoma cells. We further validated that the identified top 5 microRNAs have general differentiation-inducing effects in neuroblastoma cells with different genetic backgrounds. Furthermore, we show that miR-506-3p, the most potent novel differentiationinducing microRNA identified in our screen, shows much more potent effect on reducing cell survival than other tumor suppressive miRNAs that were previously identified in neuroblastoma cells. Further investigation shows that miR-506-3p mimic significantly reduce the ability of neuroblastoma cells to form colonies, indicating their longterm capacity to inhibit neuroblastoma cell proliferation. Altogether, these results support the therapeutic potential of the differentiation-inducing microRNAs and support that, among these microRNAs, miR-506-3p has highest therapeutic potential to be applied as a differentiation agent to treat neuroblastoma.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research