A Specific Screening Strategy to Reduce Prostate Cancer Mortality
Annual rept. 15 Aug 2013-14 Aug 2014
ALABAMA UNIV IN BIRMINGHAM
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Here, we report the use of a cancer-specific promoter, inhibition of differentiation-1 Id1, to drive a dual-reporter system Ad53- Id1-SEAP-Id1-mCherry designed for detection of prostate cancer using a blood-based reporter-secreted embryonic alkaline phosphatase SEAP and tumor visualization using a fluorescent reporter protein, mCherry. In human prostate tumors, Id1 levels are correlated with increased Gleason grade and disease progression. To evaluate in vivo performance, flank tumors were grown in athymic male mice using three prostate cancer cell lines. Following intra-tumoral injection of the vector, tumors formed by cells with high Id1 had the greatest reporter expression. Interestingly, tumors with the lowest levels of Id1 and reporter expression produced the greatest amounts of PSA. These data support the use of Ad53-Id1-SEAP-Id1-mCherry as a predictor of prostate cancer malignancy and as a strategy for tumor localization. This strategy would assist clinicians in the detection and treatment of prostate cancer and ultimately reduce the mortality associated with this disease.
- Medicine and Medical Research