Accession Number:

ADA611815

Title:

Synthesis and Characterization of DNase 1-Stabilized Gold Nanoclusters

Descriptive Note:

Final rept. Nov 2013-Jan 2014

Corporate Author:

ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD

Report Date:

2014-10-01

Pagination or Media Count:

26.0

Abstract:

DNase 1-stabilized gold nanoclusters DNase 1 AuNCs were synthesized with multiple energy levels in a metal-concentration-dependant fashion. From multiple characterization techniques including fluorescence spectroscopy, high-resolution transmission electron microscopy and X-ray photoelectron spectroscopy, we assigned 2 reaction products to contain pure populations of clusters with core sizes of 8 and 25 Au atoms for the products where 10- and 2.5-mM HAuCl4 was used in the reaction, respectively, and 2 reaction products to contain either mixtures of the Au8 and Au25 clusters or Au nanoparticles AuNPs dependent upon the initial Au concentration. An initial HAuCl4 concentration of 5 mM produced a small amount of AuNPs but no NCs, while an initial concentration of 0.5-mM HAuCl4 resulted in a mixture of Au8 and Au25 clusters with a higher percentage of Au25 clusters. The DNase 1 Au8NCs exhibiting blue fluorescence have an excitation and emission maxima of 390 and 460 nm, respectively, whereas the DNase 1 Au25NCs are red-emitting and have an excitation and emission maxima of 490 and 640 nm, respectively.

Subject Categories:

  • Atomic and Molecular Physics and Spectroscopy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE