Accession Number:

ADA611806

Title:

Preventing Prostate Cancer Metastasis by Targeting Exosome Secretion

Descriptive Note:

Annual rept. 30 Sep 2013 - 29 Sep 2014

Corporate Author:

NEW YORK UNIV NY

Personal Author(s):

Report Date:

2014-10-01

Pagination or Media Count:

7.0

Abstract:

We hypothesize that exosomes secreted by advanced prostate cancer cells groom stromal cells at pre-metastatic sites to render the bone microenvironment more favorable for metastatic growth. We propose to test the hypothesis that exosomes derived from advanced stage prostate cancer exert a pro-metastatic influence on bone stromal cells. Aim 1 We will perform a quantitative comparison of changes in the structure and cargo of exosomes derived from early versus advanced prostate cancer cell lines to identify mechanisms of action. Aim 2 We will profile alterations in potential target stromal cells upon exposure to early versus late prostate cancer exosomes to determine the effect on the metastatic microenvironment. We have made substantial progress towards establishing the protocols needed for robust and quantitative analysis. Testing this hypothesis has direct relevance towards an understanding of the often lethal complications of prostate cancer, manifested in extensive and painful metastasis of the bone.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE