Exploring the Presence of microDNAs in Prostate Cancer Cell Lines, Tissue, and Sera of Prostate Cancer Patients and its Possible Application as Biomarker
Annual rept. 1 Aug 2013-31 Jul 2014
VIRGINIA UNIV CHARLOTTESVILLE RECTOR AND VISTORS OF THE UNIVERSITY OF VIRGINIA
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MicroDNAs are extra chromosomal circular DNA present in normal mammalian somatic cells. To find the prostate tissue-specific microDNA a panel of human prostrate LnCap PSA, hK2 and AR positive, C4-2, and PC-3 non- tranformed prostate epithelium and ovarian ES2 and OVCAR-8 cancer cell lines were examined for microDNA. The identified microDNAs in all the cell lines were mostly 200bp or 400bp in size, arising from the GC rich regions in the genome, and mostly mapped to genic regions and are not associated with repetitive DNA sequences. MicroDNA are enriched in area of genome that had high exon density suggesting a role of splicing in microDNA generation. Comparison of microDNA loci across the cell lines identified hot spots of microDNA generation that are present on every chromosome, and correspond to areas of high gene density and high GC content. However, hierarchical clustering on the basis of microDNA co-ordinates classified the prostate and ovarian cancer cell lines into two separate groups suggesting that at least some microDNAs are tissue-specific and so their sites of origin are affected by tissue-specific gene expression patterns or epigenetic marks.
- Medicine and Medical Research