A Direct Quantitative Agar-Plate Based Assay for Analysis of Pseudomonas protegens PF-5 Degradation of Polyurethane Films (Postprint)
Interim rept. 14 Sep 2009-13 Sep 2014
AIR FORCE RESEARCH LAB WRIGHT-PATTERSON AFB OH FUNCTIONAL MATERIALS DIV
Pagination or Media Count:
A quantitative assay was developed for the direct measurement of polymer film degradation from bacterial colonies on agar plates. Small 1 mm diameter colonies of Pseudomonas protegens Pf-5 formerly Pseudomonas fluorescens Pf-5 were used for this work. Interactions between the Pf-5 colonies and thin polyurethane PU coatings on ZnSe coupons were evaluated for degradation using infrared spectroscopy. Three different coatings were analyzed and were formed from 1 a colloidal, aqueous e based polyester PU Impranil DLN 2 an organic solvent e based polyester PU Irogran and 3 an organic solvent e based polyether PU AS-P108. Over a 24 h time period at 30 C, citrate exposed Pf-5 cultures rapidly degraded Impranil coatings, consistent with analogous zone clearing assays. However, the Irogran and AS-P108 PUs, which are not directly compatible with zone clearing assays, showed no measureable degradation by the coating assay under identical conditions. These results demonstrated the capability to evaluate any variety of polymer formulation as solid films under identical biological conditions. The results also show that rapid microbial degradation of colloidal polyurethanes such as Impranil are not necessarily representative of activity towards other PU materials.