Chemokine Receptor Signatures in Allogeneic Stem Cell Transplantation
Annual rept. 1 Aug 2013-31 Jul 2014
TRUSTEES OF PENNSYLVANIA UNIV PHILADELPHIA PA
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The purpose of this award is to define chemokine receptor signatures that contribute to lymphocyte trafficking into target organs in human graft-versus-host disease GHVD. We use T-cell receptor deep sequencing to characterize the repertoire of effector T-cells in allogeneic hematopoietic stem-cell transplant HSCT recipients and identify the role of chemokine receptors in effector cell infiltration of target organs. In the recent funding period we studied blood and tissue samples from several allogeneic HSCT recipients and found that the clonal repertoire of CCR5 T-cells was distinct from CCR5- T-cells, implying that CCR5 is a close-specific marker and not a general activation marker. We further characterized the clonal diversity and found that the asymmetry in clonal diversity of CCR5 and CCR5- T-cells is characterized by specific V-gene usage. We are currently verifying this finding in additional patients and characterizing the presence CCR5 clones in target organs of GVHD.
- Anatomy and Physiology
- Medicine and Medical Research