Accession Number:

ADA610563

Title:

Developing and Validating Genetic Catabolic Probes for Monitored Natural Attenuation of 1,4-Dioxane with a One-Year Timeframe

Descriptive Note:

Final rept. Jul 2013-Jun 2014

Corporate Author:

RICE UNIV HOUSTON TX

Report Date:

2014-04-01

Pagination or Media Count:

76.0

Abstract:

Remediation of aquifers contaminated with 1,4-dioxane dioxane is a difficult task due to its extremely hydrophilic nature. Monitored natural attenuation MNA, which relies primarily on biodegradation, is often the most cost-effective approach to manage large and dilute groundwater plumes of primary pollutants, such as those formed by dioxane. However, improved forensic tools are needed to ensure biodegradation is occurring at impacted sites. During this one-year project, catabolic biomarkers of high selectivity and sensitivity were developed to target until now elusive dioxanetetrahydrofuran soluble di-iron monooxygenase genes using Taqman chemistry. Further, dioxane degradation activity observed in microcosms prepared with groundwater samples and aquifer materials from multiple sites was found to be significantly correlated with the abundance of thmAdxmA genes, suggesting the usefulness of this probe to assess feasibility of MNAbioremediation and estimate degradation rates.

Subject Categories:

  • Biology
  • Anatomy and Physiology
  • Inorganic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE