Accession Number:

ADA610452

Title:

Gold Nanocluster-DNase 1 Hybrid Materials for DNA Contamination Sensing

Descriptive Note:

Technical paper

Corporate Author:

ARMY RESEARCH LAB ABERDEEN PROVING GROUND MD

Report Date:

2014-01-01

Pagination or Media Count:

7.0

Abstract:

Protein encapsulated gold nanocluster P-AuNC synthesis was first demonstrated in 2009. Initially these P-AuNCs were used as cellular imaging agents as the protein shell surrounding the AuNC made them highly biocompatible. However, recent studies have begun to show that these stabilizing proteins may also retain native biological function thus giving a dual functionality to these hybrid molecules. Here we present the synthesis of DNase 1 stabilized gold nanoclusters DNase 1AuNCs with core sizes consisting either 8 or 25 atoms. The DNase 1Au8NCs exhibit blue fluorescence whereas the DNase 1Au25NCs are red emitting. Moreover, in addition to the intense fluorescence emission the synthesized DNase 1AuNC hybrid retain the native functionality of the protein, allowing simultaneous detection and digestion of DNA with a detection limit of 2 gmL Scheme 1. The DNase 1AuNCs could be conveniently employed as efficient and fast sensors to augment the current inefficient and time consuming DNA contamination analysis techniques.

Subject Categories:

  • Biochemistry
  • Physical Chemistry
  • Miscellaneous Detection and Detectors

Distribution Statement:

APPROVED FOR PUBLIC RELEASE