Accession Number:

ADA609412

Title:

Enhanced Bioactivity of Internally Functionalized Cationic Dendrimers with PEG Cores

Descriptive Note:

Journal article

Corporate Author:

CALIFORNIA UNIV SANTA BARBARA

Report Date:

2012-11-09

Pagination or Media Count:

12.0

Abstract:

Hybrid dendritic-linear block copolymers based on a 4-arm polyethylene glycol PEG core were synthesized using an accelerated AB2CD2 dendritic growth approach through orthogonal amineepoxy and thiol-yne chemistries. The biological activity of these 4-arm and the corresponding 2- arm hybrid dendrimers revealed an enhanced, dendritic effect with an exponential increase in cell internalization concomitant with increasing amine end groups and low cytotoxicity. Furthermore, the ability of these hybrid dendrimers to induce endosomal escape combined with their facile and efficient synthesis makes them attractive platforms for gene transfection. The 4-arm-based dendrimer showed significantly improved DNA binding and gene transfection capabilities in comparison with the 2-arm derivative. These results combined with the MD simulation indicate a significant effect of both the topology of the PEG core and the multivalency of these hybrid macromolecules on their DNA binding and delivery capabilities.

Subject Categories:

  • Biochemistry
  • Polymer Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE