Accession Number:

ADA609244

Title:

The Importance of Neurogenic Inflammation in Blast-Induced Neurotrauma

Descriptive Note:

Final rept. 1 Jan 2011-31 Dec 2013

Corporate Author:

JOHNS HOPKINS UNIV LAUREL MD APPLIED PHYSICS LAB

Report Date:

2014-05-01

Pagination or Media Count:

72.0

Abstract:

This study investigates the role of blood-borne immune cells as important mediators of damage and neuropathology resulting from blast-induced neurotrauma BINT. We hypothesized that macrophages, along with their secreted cytokines and chemokines from the periphery, migrate via blood and infiltrate the CNS where they contribute to neuronal damage caused by activated microglia both in acute and chronic injury phases of BINT. We have used MRI and molecular techniques in mice with mildmoderate blast injury generated in a compressed helium-driven shock tube, as well as measurements of motor performance and behavioral assessment, observed temporally over 1 month. Groups of animals with mildmoderate BINT are imaged MRI and validated by immunocytochemistry, to visualize potential macrophage infiltration blood-brain barrier BBB disturbance reactive gliosis or astrocyte activation. We show that a single exposure to mildmoderate blast induces both acute and chronic glial activation, levels of cytokineschemokines, and motor impairment.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE